fluorescens is gelatinase positive, but P. Escherichia coli ATCC 25922-No liquefaction ( Gelatin hydrolysis test negative).Pseudomonas aeruginosa ATCC 10145-Liquefaction (Gelatin hydrlysis test positive).Gelatin Hydrolysis Test Negative: At the end of the refrigeration period, the control tube and the test tube will be resolidified, even at the top of the tube as shown above figure. Note: Some organisms only partially hydrolyze gelatin or liquefy just at the surface of the tube. Gelatin Hydrolysis Test Positive: At the end of the refrigeration period, the control tube will be resolidified and the test tube will remain liquid at least to the depth of the stab. Result Interpretation for Gelatin Hydrolysis Test Reincubate a negative test for up to 2 weeks if indicated by the nature of the organism, and examined at regular intervals.Gently invert to detect liquefaction by the test organism after 30 min of refrigeration. ![]() Do not shake or invert the tubes prior to refrigeration.
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